[Tissue Engineering And Regenerative Medicine] Knocking down Nogo-B caused the downregulation of pro-fibrotic genes and inhibited the viability of hepatic stellate cells. Nogo-B knockdown prevented CCL4-induced fibrosis, accompanied by downregulation of the extracellular matrix.

[Journal Of Materials Chemistry B] A novel collagen crosslinking agent (CE) was synthesized through a reaction involving chitosan quaternary ammonium salt with 1,4-butanediol diglycidyl ether. Compared to collagen crosslinked with glutaraldehyde, the CE-crosslinked collagen implant exhibited notable stability and durability.

[Ecotoxicology And Environmental Safety] Scientists suggested that AFB1 damaged the cell morphology, induced oxidative damage and apoptosis, and degraded ECM components, in turn inhibiting myoblast development by activating the p38MAPK/urokinase-type plasminogen activator/matrix metalloproteinase/ECM signaling pathway.

[Biomedicine & Pharmacotherapy] Human pulmonary fibroblasts were transfected with miR-29a-3p, and RT-qPCR, immunostaining, and western blotting were performed to determine the expression of DNMT3A and extracellular matrix collagen-1 and fibronectin-1 levels.

[ACS Applied Materials & Interfaces] Investigators successfully validated the pivotal role of activating transcription factor 5 (ATF5) in upregulating the UPRmt, mitigating IL-1β-induced inflammation and mitochondrial dysfunction, and promoting balanced metabolism in articular cartilage ECM, proving its potential as a promising therapeutic target for osteoarthritis.

[Fierce Biotech] Europe launched the most clinical trials for cystic fibrosis treatments, while North America leads in those for acute myeloid leukemia, according to a pair of new global trial landscape reports announced by contract research organization Novotech. Global venture funding for R&D on treatments for both diseases topped $1 billion each over the past five years.

[Nature Communications] To understand the mechanistic significance of inter-regional projections on development of neural circuits, researchers investigated an in vitro neural tissue model for inter-regional connections, in which two cerebral organoids are connected with a bundle of reciprocally extended axons.

[Biomedicine & Pharmacotherapy] Scientists aimed to characterize the effect of calprotectin on mouse jejunal organoids as a model epithelium, focusing on Zn2+ metabolism and cell proliferation.

[Journal Of Neurovirology] Researchers generated and characterized several human-induced pluripotent stem cell lines from healthy human donor skin fibroblast cells. hiPSCs were then used for the generation of microglia-containing human cerebral organoids.

[Cell Reports] Scientists indicated that increasing the cellular complexity of the organoid microenvironment promoted the emergence of oRG and supported a platform to study the outer radial glia in human pluripotent stem cells-derived brain organoids routinely.

[British Journal Of Cancer] Researchers investigated the effect of trastuzumab monotherapy and its combination with different HER2 targeting treatments in a panel of breast cancer cell lines and patient-derived organoids using biochemical methods and cell viability assays.

[Familial Cancer] Researchers examined the effects of EGFR and COX pathway inhibition in organoid cultures derived from uninvolved colon and polyps of serrated polyposis syndrome patients. They also compared with organoids representing the hereditary gastrointestinal syndromes, Familial Adenomatous Polyposis and Lynch syndrome.